%0 Journal Article
%T Expression of Pili-like Protein SSU2101 of Streptococcus suis Serotype 2 and Evaluation of Its Protective Abilities to a Mouse Model<br>猪链球菌2型菌毛样结构蛋白SSU2101原核表达及其免疫保护性
%A QIN Yue-Hong
%A WANG Chang-Jun
%A DONG Rui-Ping
%A PAN Xiu-Zhen
%A TANG Jia-Qi
%A <br>秦跃红
%A 王长军
%A 董瑞萍
%A 潘秀珍
%A 唐家琪
%J 微生物学通报
%D 2009
%I 
%X To prokaryotic express pili-like protein(PLP) SSU2101 of the Chinese strain 05ZYH33 of Streptococcus suis serotype 2(S. suis 2), elucidate its immunogenicity and immunoprotection. The plp gene was amplified by PCR, and then cloned into prokaryotic expression plasmid pET32a. The recombinant plasmid was transformed into E. coli BL21 competent cells to express the recombinant PLP(rPLP). The rPLP protein was purified by affinity chromatography methods. Western blot indicated clearly that the rPLP shares strong specific immunogenicity. Animal infection experiments demonstrated that the rPLP protect mice from lethal challenge with S. suis 2 infection, which suggest rPLP might be a candidate component of S. suis 2 protein vaccine.
%K Streptococcus suis serotype 2
%K Pili-like protein
%K Immunogenicity
%K Immunoprotection<br>猪链球菌2型
%K 菌毛样结构蛋白
%K 免疫原性
%K 免疫保护作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3DDF68B85E59D5A7B97C16045EBDD63E&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=708DD6B15D2464E8&sid=19519743553AEE80&eid=030782B6ACCD7BEA&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0