%0 Journal Article
%T Effects of Phosphate-limitation on Glycerol Biosynthesis and Intracellular Phosphorus Accumulation of Candida glycerinogenes<br>限磷对产甘油假丝酵母甘油合成与胞内磷积累的影响
%A XIE Tao
%A FANG Hui-Ying
%A ZHUGE Bin
%A ZHUGE Jian
%A <br>谢 涛
%A 方慧英
%A 诸葛斌
%A 诸葛健
%J 微生物学通报
%D 2009
%I 
%X Effects of phosphate limitation on glycerol biosynthesis and intracellular phosphorus accumulation of Candida glycerinogenes were studied by recycling yeast cells. The results demonstrated that, throughout the fermentation of yeast cells recycled, intracellular phosphorus accumulation gradually decreased when yeast cells were re-inoculated from the suitable phosphate or rich phosphate medium to the low phosphate medium; while intracellular polyPhosphate accumulation rapidly increased after yeast cells were re-inoculated from the low phosphate medium to the suitable phosphate or rich phosphate medium. When yeast cells were re-inoculated from the suitable phosphate medium to the low phosphate medium at the fourteenth or thirty-eighth hour, glycerol yields on glucose of 60.9% or 61.4% could be achieved with a productivity of 2.03 g/(L·h) or 2.23 g/(L·h). These phenomena proved that it is necessary for glycerol overproduction by C. glycerinogenes to limit phosphorus concentration in fermentative medium, which provides an important basis for its glycerol production by repeated fed-batch fermentation.
%K Phosphate limitation
%K Candida glycerinogenes
%K Glycerol biosynthesis
%K polyPhosphate (polyP)<br>限磷
%K 产甘油假丝酵母
%K 甘油合成
%K 聚磷酸盐
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4795F6F6D5FAB1A89BD7CE1822C13DD8&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=9CF7A0430CBB2DFD&sid=80AD5907B47936EE&eid=B593C98A8D813A42&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11