%0 Journal Article
%T The Study of Enzymatic Activity of Mutant Strain Rhizopus oryzae with L-Malic Acid Accumulation<br>积累L-苹果酸的米根霉突变株酶活性初探
%A HE Hao
%A LI Shuang
%A XU Qing
%A FU Yong-Qian
%A HUANG He
%A <br>何 皓
%A 李 霜
%A 徐 晴
%A 付永前
%A 黄 和
%J 微生物学通报
%D 2009
%I 
%X A stable and efficient L-Malic acid accumulation mutant strain Rhizopus oryzae ME-M15 was discovered occasionally in the mutation breading for fumaric acid producers. Rhizopus oryzae ME-M15 gave a L-Malic acid output of 16.3 g/L on average after fermentation for 96 hours, more than 3 times than that of the parent strain ME-F10. In addition, other metabolites such as ethanol and fumaric acid were remarkably decreased in accordance with the depressed activity of the cytosolic isoenzyme of fumarase and alcohol dehydrogenase in strain ME-M15, while the activity of the pyruvate carboxylase had no significant difference.
%K Rhizopus oryzae
%K L-Malic acid
%K Fumarase
%K Pyruvate carboxylase (PC)
%K Alcohol dehydrogenase (ADH)<br>米根霉
%K L-苹果酸
%K 富马酸酶
%K 丙酮酸羧化酶
%K 乙醇脱氢酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=965DE39C55BAEAD5B4D811FF8ED227DD&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=38B194292C032A66&sid=8D3B992C75BB6A79&eid=B1C2C6BF6C87197E&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15