%0 Journal Article
%T Construction of the Recombinant Plasmid with esat6 Gene and Its Expression in Streptococcus gordonii<br>esat6基因表达载体的构建及其在戈登链球菌中的表达
%A JIA Ping
%A DU Xian-Zhi
%A <br>贾 平
%A 杜先智
%J 微生物学通报
%D 2009
%I 
%X To construct expressing vector carrying esat6 gene and express this protein in Streptococcus gordonii GP251. esat6 gene was amplified by PCR with specific primer from genome of Mycobacterium tuberculosis (MTB)H37Rv. Inserted esat6 into the pMD18-T vector by T/A clone to get recombinant vector pMD18-esat6. Then digested pMD18-esat6 with restriction enzyme, esat6 was cloned to vector PSMB104 and expressed in Streptococcus gordonii GP251. The expression of esat6 protein was detected by Tricine-SDS-PAGE and Western-blot, ELISA technique was also used to detect its secretory volume. Restriction endonuclease, PCR, Tric ine-SDS-PAGE and Western-blot confirmed that esat6 gene was cloned into expressing vector successfully, and a 10 kD protein secreted in Streptococcus gordonii GP251, this protein has a good immunogenicity. The expression vector of esat6 gene was constructed, and esat6 protein expressed in Streptococcus gordonii1 successfully, it will be benefit for future study.
%K Streptococcus gordonii
%K Mucosa vaccine
%K esat6 gene<br>戈登氏链球菌
%K 黏膜疫苗
%K esat6基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4D2423398AC0A98B233F6C3DC13DFB54&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=38B194292C032A66&sid=0DFB575CE62CA487&eid=7661F4B6FFD778A8&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=9