%0 Journal Article
%T Construction of Identification of Klebsiella pneumoniae in Milk and Diary Product with PCR-DHPLC<br>乳及乳制品中肺炎克雷伯氏菌PCR-DHPLC检测新技术的建立
%A YANG Chun-Hu
%A CAO Ji-Juan
%A ZHONG Yi
%A SHI Lei
%A CHEN Qing-Fu
%A MA Xin-Xin
%A GONG Fei
%A <br>杨春华
%A 曹际娟
%A 钟毅
%A 石磊
%A 陈庆富
%A 马欣欣
%A 龚斐
%J 微生物学通报
%D 2010
%I 
%X To identify the 16S?23S rRNA gene in Klebsiella pneumoniae, a PCR-DHPLC assay was performed in this study. Primers specific for the 16S?23S rRNA gene of Klebsiella pneumoniae in diary were selected to conduct the DHPLC assays. The specific testing was performed with Klebsiella pneumoniae and 57 strains, and various grads of Klebsiella pneumoniae was used for the sensitivity testing. The good specificity was found in this study. Also, the method showed nice sensitivity with the lowest amount of detecting at 100 CFU/mL, so it can detect and identify Klebsiella pneumoniae quickly and correctly. The DHPLC could be a new method for detecting diary pathogens.
%K DHPLC
%K Klebsiella pneumoniae
%K 16S?23S rRNA<br>DHPLC
%K 肺炎克雷伯氏菌
%K 16S?23S
%K rRNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=D3E281FDF6EAE1FC0049D8E7F77383B5&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=59906B3B2830C2C5&sid=EFCEB529E333AB35&eid=A369E079A2D86222&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0