%0 Journal Article
%T OPTIMIZATION OF TRANSFORMATION SYSTEM OF STREPTOMYCES AUREOFACIENS<br>金色链霉菌转化系统的优化
%A MENG Chun
%A GUO Yang-Hao
%A YE Qin
%A SHI Xia-Ai
%A CHEN Jian-Feng
%A <br>金色链霉菌转化系统的优化
%J 微生物学通报
%D 2002
%I 
%X 12% sucrose, 0.5% glycin and 1 hour enzymolysis of lysozyme were the optimal conditions for preparation of Streptomyces aureofaciens protoplast. The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium. P-buffer was better than T-buffer in plasmid transformation process. 33% PEG1000 was the fit concentrations for plasmid transformation.
%K Protoplast of Streptomyces aureofaciens
%K Bran medium
%K Plasmid transformation<br>金色链霉菌原生质体
%K 麸皮培养基
%K 质粒转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7A4B3C0CFFEB5ED8&yid=C3ACC247184A22C1&vid=771469D9D58C34FF&iid=94C357A881DFC066&sid=F3583C8E78166B9E&eid=BCA2697F357F2001&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=4