%0 Journal Article
%T Selection of Reference Genes for Real-time Quantitative PCR<br>实时荧光定量PCR中内参基因的选择
%A ZHAO Wen-Jing
%A XU Jie
%A BAO Qiu-Hu
%A CHEN Yong-Fu
%A ZHANG He-Ping
%A <br>赵文静
%A 徐洁
%A 包秋华
%A 陈永福
%A 张和平
%J 微生物学通报
%D 2010
%I 
%X Real-time quantitative PCR is a commonly used method to analyse the gene expression profile, it is important to select an appropriate reference gene for normalization of experimental data when using this method. In our study, we used two statistical methods to evaluate the gene expression stabilities of five reference genes (ldh, recA, rpoB, gapdh and 16S rRNA) under the different growth phases of Lactobacillus helveticus H9. The results showed that the best reference gene was ldh which was the most stable gene would be used for normalization of real-time quantitative PCR experiments data.
%K Real-time quantitative PCR
%K Reference genes
%K GeNorm
%K NormFinder<br>实时荧光定量PCR
%K 内参基因
%K GeNorm
%K NormFinder
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=36465D79D8F2A7B2E7CD8B4953C1965B&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=59906B3B2830C2C5&sid=BD667367F07B7EA7&eid=3381B1AF30824ACC&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0