%0 Journal Article
%T Isolation, Preservation, and Species Identification of a Toxigenic Strain of Microcystis from Chaohu Lake<br>巢湖微囊藻产毒株的分离、保存及种属特异性鉴定
%A LIU Yang
%A ZHA Xiang-Dong
%A LI Yu-Cheng
%A <br>刘杨
%A 查向东
%A 李玉成
%J 微生物学通报
%D 2010
%I 
%X We isolated and purified a Microcystis strain, Chaohu-1, from Chaohu Lake by the method of plate streaking and liquid culture. After the successive steps of methanol extraction and solid-phase ex-traction, it was confirmed that the algae strain produces MC-LR as analyzed by HPLC. The algae cells were green and spherical, while the population displayed a random reticulate form. The phycocyanin intergenic spacer region (PC-IGS) was amplified by whole-cell PCR, cloned and sequenced. The PC-IGS sequence showed a degree of similarity of 99% with those of Microcystic aeruginosa strains downloaded from GenBank. A combination of the analysis results both at morphological and molecular levels indicated that for the first time we have isolated a Microcystis aeruginosa strain in Chaohu Lake, and we named it Chaohu-1. Moreover, we established an effective method for its cryopreservation and cell resuscitation, which laid foundation for the long-term preservation and the future research.
%K Microcystis aeruginosa
%K Isolation
%K Toxigenic
%K Species identification
%K Cryopreservation and resuscitation<br>铜绿微囊藻
%K 分离纯化
%K 产毒特性
%K 种属鉴定
%K 冻存复苏
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=EECB1A649033392C1FD1F7FD7C3853BD&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=59906B3B2830C2C5&sid=09614E07E1BC4184&eid=7C95AAC7652395EE&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0