%0 Journal Article
%T PREPARATIVE SEPARATION OF HMC-TOXIN FROM HELMINTHOSPRIUM MAYDISRACE C BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMOTOGRAPHY<br>反相高效液相色谱法制备HMC毒素纯品
%A Cui Yang
%A Chen Yongli
%A Zhao Nanming
%A <br>崔洋
%A 陈永丽
%A 魏建昆
%A 赵南明
%J 微生物学通报
%D 1999
%I 
%X 本文采用反相高效液相色谱纯化制备了从玉米小斑病病菌Ｃ小种（ＨｅｌｍｉｎｔｈｏｓｐｒｉｕｍｍａｙｄｉｓＲａｃｅＣ）分离出来的毒素ＴｏｘｉｎＩ，经ＮＭＲ的结构分析证实此制备物纯度较高，达到结构分析要求。用氯仿提取含有ＨＭＣ的毒素培养滤液，经过多次ＴＬＣ展层层析（ＣＨＣｌ_３：ＭｅＯＨ＝９：１），刮取具有侵染活性的部分（Ｒｆ＝０．４）溶解在小体积甲醇中备用。色谱柱为Ｗａｔｅｒｓ（制备型２５０ｍｍ×１０ｍｍ）填料为ＹＷＧ－Ｃ１８反相柱，粒度１０ｕｍ，流动相选用甲醇：水５５：４５
%K High performance liquid chromatography
%K Preparation
%K HMC toxin<br>高效液相色谱
%K 制备
%K HMC毒素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0B100A5EC19CFD1EC8B30B6E7DB0AF42&yid=B914830F5B1D1078&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=F4B561950EE1D31A&eid=2A3781E88AB1776F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=5