%0 Journal Article
%T Effect of Conditioned Mediums of Astrocytes on Activity of AKP and γ-GT from Rat Brain Microvascular Endothelial Cells<br>不同状态的大鼠星形胶质细胞条件培养液对脑微血管内皮细胞血脑屏障特征性酶的影响
%A Zhu Yuan
%A Li Pengtao
%A Li Weihong
%A Jia Jing
%A Gai Cong
%A Liu Yang
%A <br>朱 元
%A 李澎涛
%A 李卫红
%A 贾 静
%A 盖 聪
%A 刘 洋
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X This study was aimed to observe the effect of conditioned media from astrocytes on the rat brain microvascular endothelial cells(BMEC),including the activity of BMEC,AKP and γ-GT,as well as to investigate the influence of Tong Luo Jiu Nao(TLJN) injection.The conditioned media from normal,activated,activated and TLJN administrated,injured,injured and TLJN administrated astrocytes were collected.Then 5 conditioned media were added into injured BMEC cultures respectively.The effect was determined through the measurement of MTT and AKP,γ-GT Kit.The results showed that conditioned medium from normal astrocytes significantly increased the activity of BMEC,AKP and γ-GT.The conditioned medium from activated astrocytes showed similar function,but it was weaker than normal astrocytes.The injured astrocytes conditioned medium did not show any function on injured BMEC.In the groups treated with TLJN,BMEC activity and the expression of AKP,γ-GT was higher than groups without treatment.It was concluded that the secretion of normal astrocytes can highly increase the activity of BMEC,AKP and γ-GT.As the astrocytes were activated and injured,this function would be decreased or disappeared.TLJN can observably improve the function of astrocyte on the activity of BMEC,AKP and γ-GT,which may be the mechanism of TLJN on the protection of blood-brain barrier.
%K Conditioned media from astrocytes
%K rat brain microvascular endothelial cells
%K AKP
%K γ-GT
%K Tong Luo Jiu Nao injection<br>星形胶质细胞条件培养液
%K 脑微血管内皮细胞
%K AKP
%K γ-GT
%K 通络救脑注射液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=4561954BE0A0F99D1BA255B5C3BDBFD9&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&sid=532CB3FA5DC08D32&eid=C4160F9DDB7598AD&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0