%0 Journal Article
%T Molecular Identification of Sophorae Tonkinensis Radix et Rhizoma Original Plant and Its Adulterants based on ITS2 DNA Barcode<br>基于ITS2条形码序列的山豆根基原植物及其混伪品的DNA分子鉴定
%A Xu Xiaolan
%A Shi Linchun
%A Song Jingyuan
%A Han Jianping
%A Yao Hui
%A Chen Shilin
%A He Shunzhi
%A <br>徐晓兰
%A 石林春
%A 宋经元
%A 韩建萍
%A 姚 辉
%A 陈士林
%A 何顺志
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X In order to discriminate Sophorae tonkinensis Radix et Rhizoma original plant and its adulterants, we collected 84 ITS2 sequences of Sophorae tonkinensis Radix et Rhizoma original plant and its adulterants belonging to 38 species from 9 distinct genera of five families and compared the divergence of these sequences. Then, the genetic distances were computed using PAUP 4b10 in accordance with the Kimura 2-Parameter (K2p) model, and the phylogenetic tree was constructed using the neighbor-joining and Kimura 2-parameter methods. The results showed that ITS2 sequences from different Sophorae tonkinensis samples in this study are identical. ITS2 sequences between Sophorae tonkinensis and its closely related species have 102 variant sites and their mean K-2p distance is 0.072. ITS2 sequences between Sophorae tonkinensis and its other adulterant species have 200 variant sites and their mean K-2p distance is 0.594. Therefore, ITS2 can be used to correctly identify Sophorae tonkinensis Radix et Rhizoma original plant and its adulterants.
%K Sophorae tonkinensis Radix et Rhizoma
%K DNA barcoding
%K Adulterants
%K ITS2
%K Identification<br>山豆根
%K 越南槐
%K 混伪品
%K ITS2
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=0DFF8C17EFCA83331EB6225F0CD182C5&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=CA4FD0336C81A37A&sid=008520E0B52E94B3&eid=A3A7F1D1E8071003&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0