%0 Journal Article
%T Numerical taxonomy of rhizobia isolated from the nodules of Sophora alopecuroides from Xinjiang<br>新疆苦豆子根瘤菌的数值分类研究
%A Mingfu Gong
%A Gehong Wei
%A Jianghua Liu
%A Peng Su
%A Peng Cao Northwest Sci-Tech University of Agriculture
%A Forestry
%A Yangling
%A Shaanxi College of Plant Science
%A Technology
%A Tarim University
%A Alar
%A Xinjiang
%A <br>龚明福
%A 韦革宏
%A 刘江华
%A 苏鹏
%A 曹鹏
%J 生物多样性
%D 2005
%I Science Press
%X We collected the nodules of Sophora alopecuroides from a number of locations in Xinjiang, China. From these nodules, we isolated 67 previously unknown strains of rhizobia. We analyzed the phenotypic characteristics and the numerical taxonomy of these rhizobia and 36 reference strains through 118 parameters. The results showed that the strains differed in some characteristics, including carbon and nitrogen source, resistance to antibiotics and chemical dye, and endurance to salt and alkali. All unknown strains were tolerant to low temperature, and grew in YMA medium with an initial pH from 9 to 12. Up to 92.5% of the strains were able to grow on YMA medium containing 3.0% NaCl, and more than 91.0% were able to grow on YMA medium containing 4.0% NaCl. Eighteen strains were able to tolerate NaCl concentrations as high as 5%-6% in the growth medium. The unknown strains formed four new clusters defined by at least 84.8% similarity. Cluster I had 21 strains and NWBC152 was the central strain. Cluster II had 7 strains and NWTKX101 was the central strain. Cluster III had 4 strains and NWYJS12 was the central strain. Cluster IV had 3 strains and NWLP112 was the central strain. In addition, the numerical taxonomic analysis indicates that some strains in the new clusters could be new taxa.
%K Sophora alopecuroides
%K phenotypic characteristics
%K cluster analysis
%K biological nitrogen fixation<br>Sophora
%K 表型性状
%K 聚类分析
%K 生物固氮
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=284A3BE2D09272C2DA95716775015427&aid=0034D3B2F3678EAE&yid=2DD7160C83D0ACED&vid=FC0714F8D2EB605D&iid=CA4FD0336C81A37A&sid=80A07035DF96B0C4&eid=E203FB1A272C9DD2&journal_id=1005-0094&journal_name=生物多样性&referenced_num=7&reference_num=11