%0 Journal Article
%T Impairment of Protein Trafficking upon Overexpression and Mutation of Optineurin
%A BumChan Park
%A Hongyu Ying
%A Xiang Shen
%A Jeong-Seok Park
%A Ye Qiu
%A Rajalekshmy Shyam
%A Beatrice Y. J. T. Yue
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0011547
%X Glaucoma is a major blinding disease characterized by progressive loss of retinal ganglion cells (RGCs) and axons. Optineurin is one of the candidate genes identified so far. A mutation of Glu50 to Lys (E50K) has been reported to be associated with a more progressive and severe disease. Optineurin, known to interact with Rab8, myosin VI and transferrin receptor (TfR), was speculated to have a role in protein trafficking. Here we determined whether, and how optineurin overexpression and E50K mutation affect the internalization of transferrin (Tf), widely used as a marker for receptor-mediated endocytosis.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011547