%0 Journal Article
%T Effect of Petroleum Hydrocarbons on DNA Damage of Hemolymph Cells in Scallop Chlamys farreri : A Preliminary Research<br>石油烃对栉孔扇贝血淋巴细胞DNA损伤的初步研究
%A Wang Xiaoyan
%A Jiang Fenghu
%A Feng Lijuan
%A Gao Wei
%A Zhao Meili
%A Zheng Li
%A <br>王晓艳
%A 蒋凤华
%A 冯丽娟
%A 高伟
%A 赵美丽
%A 郑立
%J 生态毒理学报
%D 2012
%I 
%X To investigate the toxic effects of petroleum hydrocarbons on marine organisms,Chlamys farreri were exposed to petroleum hydrocarbons at three different concentrations (0.08, 0.21 and 0.88 mg·L-1), and the concentration of petroleum hydrocarbons in control group was 0.04 mg·L-1. The DNA damage of hemolymph cells in Chlamys farreri was detected by single cell gel electrophoresis (comet assay) after different exposure times. Results showed that the DNA damage in hemolymph cells of Chlamys farreri was induced by low concentration of petroleum hydrocarbons (0.08 mg·L-1) after short-time exposure (<7d). The DNA damage degree increased with the increase of petroleum hydrocarbon concentration and the extension of exposure time. The DNA damage induced by petroleum hydrocarbons was very serious under the concentration of 0.88 mg·L-1. After 3 days recovery, the DNA damage recovered to different degrees in all treatment groups. The comet assay was proven to be a useful tool for detecting DNA damage induced by petroleum hydrocarbons in marine mussels. It is demonstrated that DNA damage in hemolymph cells of mussels was a promising biomarker of petroleum hydrocarbons, and could be used in early warning and monitoring of marine pollution.
%K petroleum hydrocarbon
%K Chlamys farreri
%K hemolymph cells
%K DNA damage
%K single cell gel electrophoresis (SCGE)
%K comet assay<br>石油烃
%K 栉孔扇贝
%K 血淋巴细胞
%K DNA损伤
%K 单细胞凝胶电泳
%K 彗星实验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=BEC1F4700DD66C14D137590A0896A0AD&jid=B98D390DF92C35E6DD1987675E4EE86E&aid=0B7F1007E8CE29E0FD5FE192D1DACD4D&yid=99E9153A83D4CB11&vid=DF92D298D3FF1E6E&iid=38B194292C032A66&sid=407C905D8F0449C4&eid=A2745AA1110798CA&journal_id=1673-5897&journal_name=生态毒理学报&referenced_num=0&reference_num=0