%0 Journal Article
%T RAPD variation within and among four populations of Paris polyphylla<br>多叶重楼遗传多样性的RAPD分析
%A ZHANG Jin-Yu
%A YU Hong
%A ZHANG Shi-Gang
%A DING Chang-Chun Laboratory of Ecological Genetics
%A College of Life Science
%A Yunnan University
%A Kunming Inmol Laboratory of Biotechnology of Yunnan
%A Kunming Agricultural Quality
%A Standard
%A Testing for Technology Institute
%A Yunnan Academy of Agricultural Sciences
%A Kunming
%A <br>张金渝
%A 虞泓
%A 张时刚
%A 丁长春
%J 生物多样性
%D 2004
%I Science Press
%X The randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic diversity and structure of four populations of two varieties of Paris polyphylla. As a control, a population of P. cronquistii was compared with the four P. polyphylla populations to determine their phylogenetic relationships. Using 16 random primers, a total of 246 RAPD polymorphic loci were detected. At the population level, the percentage of polymorphic loci (PPB) of two populations of P. polyphylla var. yunnanensis was 57.4% and 54.67%, while Shannon information index (I) was 0.3080 and 0.2830, respectively. The PPB of two P. polyphylla var. chinensis populations was 56.33% and 57.75%, while Shannon information index (I) were 0.3080 and 0.3293, respectively. At the variety level, the PPB, I and Gst values of P. polyphylla var. yunnanensis were 75.14%, 0.3922, and 0.3085, respectively, while those of P. polyphylla var. chinensis was 80.31%, 0.3992, and 0.3726, respectively. At the species level, the PPB was 92.05%, and Gst was 0.5151. The UPGMA dendrogram showed that the two varieties of P. polyphylla are more related to each other than to P. cronquistii, which supports the previous treatment of P. polyphylla var. yunnanensis and P. polyphylla var. chinensis as two varieties within P. polyphylla based on gross morphology.
%K Paris polyphylla
%K RAPD
%K genetic diversity
%K population genetic differentiation<br>Paris
%K polyphylla
%K RAPD
%K 遗传多样性
%K 居群遗传分化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=284A3BE2D09272C2DA95716775015427&aid=B5FF9A4C3AA0DDA4&yid=D0E58B75BFD8E51C&vid=59906B3B2830C2C5&iid=94C357A881DFC066&sid=5A751AE9FA58A3FB&eid=91BAD12CFABB3251&journal_id=1005-0094&journal_name=生物多样性&referenced_num=10&reference_num=17