%0 Journal Article
%T A preliminary study on conservation of Andrias davidanus semen in vitro<br>大鲵精液离体保存的初步研究
%A ZHAO Hu
%A ZHANG Hong-xing
%A WANG Qi-jun
%A CHEN Jiu-an
%A <br>赵虎
%A 张红星
%A 王启军
%A 陈玖安
%J 水生态学杂志
%D 2012
%I 
%X Gonadal development of Andrias davidanus not synchronized,semen characteristics and preservation conditions of A.davidanus were studied targeting the increase of fertility rate.Semina were collected by artificial assistance approach,and a regular assay was conducted on the basic features such as semen volume,pH,density,vitality,etc.Preservation conditions,namely,protection medium,illumination and temperature,were also studied.The results showed that semen volume of pro-male A.davidanus was up to 12 mL/kg,pH 6.7-7.0 of subacid condition;and that the semen density was(2.15-10.75)million/mL,sperm motility 0.7-0.9.In vitro conservation conditions,dark environment at ambient illuminance(20 lx).The result also indicated that the survival time of the semina was 0.5 h longer than those conserved in the luminous environment(400 lx);low temperature(0-4℃) could extend the survival time of the semina 3d longer than those at room temperature(21-22℃) in dark environment;in the cold dark environment,semina with protection medium added in could extend the survival time 6-12 h longer than the control group.
%K Andrias davidanus
%K semen
%K conservation in vitro<br>大鲵(Andrias
%K davidianus)
%K 精液
%K 离体保存
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=A129AB11A5573C59&jid=AD42C12863085B5F664F8056EF6D57C2&aid=E80287434310BE2D2D9F574E59A299E6&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=EB552E4CFC85690B&eid=04445C1D2BDA24EE&journal_id=1674-3075&journal_name=水生态学杂志&referenced_num=0&reference_num=13