%0 Journal Article
%T Structure Alignments of Zebrafish p53 Gene and Its Application in Ecotoxicology<br>斑马鱼p53基因结构比对分析及其在生态毒理学中的应用
%A GU Ying
%A LI Yan
%A ZHAO Wei-feng
%A YIN Da-qiang
%A ZHAO Qing-shun
%A <br>顾颖
%A 李延
%A 陈蔚丰
%A 尹大强
%A 赵庆顺
%J 生态毒理学报
%D 2006
%I 
%X Zebrafish p53 gene structure and amino acid sequence conservation of twelve vertebrates were analyzed. The results showed that zebrafish p53 gene comprised 11 exons and 10 introns. Exon 1 and part of exon 11 encode untranslated region (UTR)of mRNA. The p53 cDNA was 2089 bp long containing an open reading frame (ORF)of 1122 bp. This ORF encoded a polypeptide with 373 amino acids. The 5'-UTR and 3'-UTR were 43 bp and 924 bp respectively. Protein sequence alignments of p53 gene among 12 vertebrates including human showed a remarkable conservation in several domains, which had been shown to be critical for p53 function. The research indicated that zebrafish p53 gene could be used as a molecular model to evaluate the genotoxicity of environmental chemicals in the future.
%K zebrafish
%K p53 gene
%K environmental carcinogens
%K ecotoxicology<br>斑马鱼
%K p53基因
%K 环境致突变物
%K 生态毒理学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=BEC1F4700DD66C14D137590A0896A0AD&jid=B98D390DF92C35E6DD1987675E4EE86E&aid=8524705DC8235CB8&yid=37904DC365DD7266&vid=CA4FD0336C81A37A&iid=CA4FD0336C81A37A&sid=94E7F66E6C42FA23&eid=987EDA49D8A7A635&journal_id=1673-5897&journal_name=生态毒理学报&referenced_num=0&reference_num=27