%0 Journal Article
%T Expression Changes of Apoptosis Related Genes after Pentachlorophenol Exposure and Molecular Cloning and Phylogenetic Analysis of caspase-2 in Zebrafish<br>五氯酚暴露诱导斑马鱼胚胎细胞凋亡相关基因的变化及caspase-2基因克隆和系统进化分析
%A ZHU Han-kai
%A WU Zhao-yi
%A ZHAO Qing-shun
%A YIN Da-qiang
%A <br>朱含开
%A 吴兆毅
%A 赵庆顺
%A 尹大强
%J 生态毒理学报
%D 2008
%I 
%X Employing microarray analysis, we found that 14 apoptosis related genes including caspase-2 changed their expressions after pentachlorophenol exposure to zebrafish embryos. Using bioinformatics, we modeled the phylogenetic tree of these functional genes and made phylogenetic analysis on caspase-2 gene, related to apoptosis induced by toxicants. Zebrafish caspase-2 comprises 10 exons and 9 introns. The caspase-2 cDNA is 1308bp long and contains an open reading frame predicted to encode a protein with 435 amino acids. Protein sequence alignments and phylogenetic analysis among 6 vertebrates showed Caspase-2 proetein is highly conserved during evolution. Performing RT-PCR, zebrafish caspase-2 cDNA was cloned and confirmed. Taken together, our results indicated that zebrafish caspase-2 gene could be used as a model molecular to evaluate the effect of toxicants on apoptosis.
%K bcaapase-2基因
%K 斑马鱼
%K 细胞凋亡
%K 进化树
%K 五氯酚
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=BEC1F4700DD66C14D137590A0896A0AD&jid=B98D390DF92C35E6DD1987675E4EE86E&aid=7974DB571A636A2AC91A7DD8F00E83BD&yid=67289AFF6305E306&vid=38B194292C032A66&iid=E158A972A605785F&sid=35E8A259891FB32F&eid=FA3423FC1AE95C4E&journal_id=1673-5897&journal_name=生态毒理学报&referenced_num=2&reference_num=16