%0 Journal Article %T A CONFORMATIONAL CHANGE OF BACTERIORHODOPSIN CAUSED BY PAPAIN DIGESTION
酶切菌紫质C端引起的紫膜蛋白的发光性质变化 %A 曹毅 %A 李庆国 %A 程极济 %J 生物物理学报 %D 1986 %I %X Bacteriorhodopsin shows an unnormal fluorescence emission after removing the C-terminal region by papain digestion in low concentration. The Trp fluorescence intensity is enhanced and shows a red shift at high salt concentrtion of KC1. The quenching effect of Cs+ on Trp fluorescence suggests that some Trp residues have exposed to the medium. The increasing intensity of phosphorescence emission of papain-treated bR indicates also the Trp exposure to the medium. The possible effect of the conformational change on proton pumping is discussed. %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=89960DC9CD57001BA8DE28947B095D53&yid=4E65715CCF57055A&vid=0B39A22176CE99FB&iid=E158A972A605785F&sid=4FE459D71E3BF8EB&eid=9F8C5EF901EA1E7E&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0