%0 Journal Article
%T Proteomics Analysis of Cerebral Cortex from Wistar Rat<br>大鼠脑皮质表达蛋白质组学研究
%A ZHAO Xiao-feng
%A WEN Jing-rong
%A WANG Shu
%A SHI Xue-min
%A <br>赵晓峰
%A 温景荣
%A 王舒
%A 石学敏
%J 生物物理学报
%D 2007
%I 
%X To analyze the protein expression style in Wistar rat cerebral cortex using the proteomics approach.Proteins in Wistar rat cerebral cortex were separated by two-dimensional gel electrophoresis.The protein spots in two-dimensional gel electrophoresis map were digested with trypsin,and identified using a matrix-assisted laser desorption/ionization time of flight mass spectrometry.108 protein spots were identified,including metabolism enzymes,skeleton proteins,heat shock proteins,antioxidant proteins,signaling proteins,proteasome related proteins,neuron and glial specific proteins,serum associated proteins,et al.The investigation presented was a first step towards a systematic analysis of the proteome of the rat brain.This approach may lay a foundation for the further investigation of pathogenic progress in rat models of neurological disorders.
%K Brain proteomics
%K Two-dimensional gel electrophoresis(2-DE)
%K Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS)<br>脑蛋白质组学
%K 双向凝胶电泳
%K 基质辅助激光解吸/电离飞行时间质谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=662540567D7B5FB3&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=771469D9D58C34FF&eid=7C3A4C1EE6A45749&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=3&reference_num=11