%0 Journal Article
%T AN IMPROVED METHOD FOR DATA ANALYSIS OF FLUORESCENCE DECAY MEASUREMENT<br>时间分辨荧光光谱测量结果的一种数据处理改进方法
%A 李湘正
%A 郭础
%J 生物物理学报
%D 1990
%I 
%X By combing the pr.nciples of the methods of moments and Laplace transfo-rms an improved method for data analysis of fluorescence decay curve measured by time-correlated single photon counting technique was presented. This method enables us to analyze the experimental data with an accuracy compared to those of the least squares fitting, but in a less time-consuming manner. This advantage of the presented method has been demonstrated by an analysis of bi-expo-nential fluorescence decay observed in ethanolic solution of Coumarin 102+ DCM as wall as that of Coumarin 102 + PBBO laser dye mixtures. In this paper the correction for instrumental time shift and the integration of truncated data required in Laplace transforms were also discussed.
%K 时间分辨光谱
%K 光谱测量
%K 数据处理法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=33065D87B9A63FA3AEF1DA2C893B6897&yid=8D39DA2CB9F38FD0&vid=B31275AF3241DB2D&iid=38B194292C032A66&sid=73648F51F187AC5E&eid=9C82B18080268586&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=1