%0 Journal Article
%T DNA Damage of Germ Cell in Rats Induced by Nitrotoluenes<br>硝基甲苯对小鼠睾丸生殖细胞DNA的损伤作用
%A YANG Li
%A XU Jing-bo
%A SHENG Lian-xi
%A MA Xun-feng
%A SUN Zhi-wei
%A SHI Long
%A <br>杨丽
%A 徐镜波
%A 盛连喜
%A 马逊风
%A 孙志伟
%A 石龙
%J 生态毒理学报
%D 2006
%I 
%X In order to provide a scientific basis for environmental reproductive toxicology and the culture of germ cell of fish, we study the DNA damage of germ cell of male rat induced by 2,4-DNT, 2,6-DNT and 4-NT using sertoli/germ cell cocultures and comet assay, and also discuss the reproductive toxicology of these three chemicals. The results show that all three nitrotoluene compounds tested(2,4-DNT, 2,6-DNT, 4-NT)could induce DNA strand breaks with significant logarithmic dose-effect relationship. What's more, these three chemicals are different in the degree of DNA damage to germ cell of male rat. The sequence is 2,6-DNT>2,4-DNT>4-NT, indicating the toxicity of dinitrotoluenes is higher than mononitrotoluene. There is significant(p<0.01, p<0.05)difference between the damage rate of these treated groups and that of PBS control, suggesting that 2,4-DNT, 2,6-DNT, 4-NT have geontoxicity and can induce the germ cell DNA strand to break in vitro.
%K 2
%K 4-DNT
%K 2
%K 6-DNT
%K 4-NT
%K sertoli/germ cell culture
%K comet assay
%K DNA damage<br>2
%K 4-DNT
%K 2
%K 6-DNT
%K 4-NT
%K 支持细胞/生殖细胞共培养法
%K 彗星实验
%K DNA损伤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=BEC1F4700DD66C14D137590A0896A0AD&jid=B98D390DF92C35E6DD1987675E4EE86E&aid=3C7D0584022C26EB&yid=37904DC365DD7266&vid=CA4FD0336C81A37A&iid=E158A972A605785F&sid=358F98408588E522&eid=F637763636425CAF&journal_id=1673-5897&journal_name=生态毒理学报&referenced_num=0&reference_num=25