%0 Journal Article
%T THE INTERPRETATION METHODS OF DATABASE SEARCH RESULTS IN PROTEOMICS REASEARCH: USING ION TRAP TANDAM MASS SPECTROMETRY<br>蛋白质组研究中离子阱串联质谱数据搜库结果解释方法
%A ZHANG Ji-yang
%A ZHU Yun-ping
%A XIE Hong-wei
%A HE Fu-chu
%A <br>张纪阳
%A 朱云平
%A 谢红卫
%A 贺福初
%J 生物物理学报
%D 2006
%I 
%X Shotgun method based on ion trap tandem mass spectrometry is a high-thought protein identification strategy in proteomic research. The data is usually processed by searching protein sequence database with software SEQUEST. Each spectrum is assigned some peptides with some scores. In order to determine the identified proteins, filteration and false positive rate estimation should be performed on the peptide identifition results, and then the protein list can be inferred. The two problems have no perfect solution currently. Reviewing the method can provide a reference of the strategy selection and be helpful for the data quality control.
%K SEQUEST<br>离子阱串联质谱
%K 数据质量控制
%K 蛋白质鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=D35C6B3B37FF989C&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=E158A972A605785F&sid=5B5B75F4854B8331&eid=1D01216AD76577EC&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=1&reference_num=46