%0 Journal Article
%T MASS LOSS OF QUANTITATIVE ELECTRON PROBE X RAY MICROANALYSIS FOR BIOLOGICAL THIN SPECIMEN<br>定量电子束显微分析中生物薄样品的质量损失
%A HUANG Yao
%A YU Zhang
%A FA Jing
%A YU Yong-fu
%A LING Yi-ping
%A <br>黄耀
%A 俞彰
%A 法京
%A 俞永富
%A 凌诒萍
%J 生物物理学报
%D 2002
%I 
%X The O Kα peak counts and continuum counts within 4.2-6.2 keV were used to monitor the mass loss extent of thin biological ultra cryosections and standards during electron probe X ray microanalysis respectively, these results show that the O Kα peak counts declines more steeply than continuum counts(P<0.05). Under the same electron irradiation, not all the samples lose mass to the same extent(P<0.05). Cultured liver carcinoma cell dried cryosections, gelatin dried cryosections, BSA films, aminopastic thin sections, eryothrocyte cryosections and phosvitin films lose 33%,28%,26%,18%,13% and 13% respectively. These results suggest that (1) the O Kα peak counts decrease is more sensitive than continuum counts alteration, (2) the mass loss extent should be corrected during quantitative electron probe X ray microanalysis.
%K Quantitative X-ray microanalysis
%K Standards
%K Mass lose
%K Biological thin sections<br>定量电子束显微分析
%K 生物样品
%K 质量损失
%K 电镜X－射线显微定量分析
%K 质量损失
%K 薄试样
%K 标样
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=729B0052F518A7F4&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=B344543C2864D684&eid=7C72DBC13F2D71EC&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=9