%0 Journal Article
%T PHOTOSENSITIZING DAMAGE OF PORPHYRIN-LIKE EARTH COMPLEX(ES) (PLM-Gd) TO MURINE ASCITIC HEPATOMA CELLS<br>类卟啉稀土配合物对于小鼠腹水肝癌细胞光敏损伤的研究
%A 乐加昌
%A 庞素珍
%A 孙文芳
%A 董世明
%A 王夺元
%J 生物物理学报
%D 1994
%I 
%X The porphyrin-like macromolecules and their rare earth metal complexes (PLM-Complex) belong to a noval family of dye molecules, they absorb strongly around 760-780nm, which were first suggested as potential photosensitizers for Photo Dynamic therapy (PDT) by J.L. Sessler in 1987. Up to now their photo sensitizing damage to cells have not been studied. In this paper we studied the PLM-Gd photosensitized cytotoxity to Murine ascitic hepatoma cell(AH). The results showed that the cellular uptake of PLM -Gd is a complicated process. The saturation uptake concentration is aboat 1× 109 molecular/each each cell of AH cells. The cell mortality was proportional to PLM-Gd concentration upon illumination at 760 nm wavelength. The ability to kill AH cell among the PLM-Gd;PLM-Eu; PLM-Sm and PLM-Gd-B are in the following order: PLM-Gd-B;PLM -Gd-A; PLM- Eu; PLM -Sm. With Fluorometric analysis of DNA unwinding method for detection of DNA strand breaks in cell, the results showed that DNA strand breaks remarkably. By using 1O2 quenchers(L-His,NaN3), superoxide anion radicals quencher (SOD), we proved that singlet oxygen and surperoxide anion were involved in the process of PLM-Gd photosensitized cytotoxicity .
%K Porphyrin-like earth complex Photosensitization Murine Hepatoma cells<br>类卟啉
%K 稀土配合物
%K 光敏作用
%K 腹水
%K 肝癌细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=921269908311B5AE79AA194D8C76F374&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=E158A972A605785F&sid=20ED669EB429E15C&eid=DB7B2C790D19BE6E&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=7&reference_num=4