%0 Journal Article
%T PREPARATION AND OSTEOBLAST AFFINITY OF beta-TRICALCIUM PHOSPHATE /APATITE COMPOSITES<br>多孔beta-磷酸三钙与磷灰石复合材料的制备及其细胞相容性研究
%A GAO Yuan
%A XI Jing
%A DONG Li-min
%A GONG Yan-dao
%A TIAN Jie-mo
%A ZHAO Nan-ming
%A ZHANG Xiu-fang
%A <br>高媛
%A 奚静
%A 董利民
%A 公衍道
%A 田杰谟
%A 赵南明
%A 张秀芳
%J 生物物理学报
%D 2005
%I 
%X A biomimetic process was developed to prepare two groups of -tricalcium phosphate/ apatite composites, sample 1 and sample 2. First, porous -tricalcium phosphate scaffold material was prepared by a multiplex method, which integrated foam technique in porous ceramics preparing and three-dimensional gel-lamination technique in rapid prototyping. Subsequent immersion in a solution with 1.5 times ion concentrations of simulated body fluid (1.5 SBF) led to the formation of sample 1. Different from sample 1, the preparation of sample 2 involved a pre-soaking of sodium hydroxide (NaOH) solution before immersion in 1.5 SBF. They were characterized by scanning electron microscopy (SEM), mercury intrusion porosimetry, wide angle X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FT-IR). Further, MTT assay was used to investigate the attachment and proliferation of mouse preosteoblastic cells cultured on the composites. The cells morphology on the materials was observed by SEM. XRD and FT-IR measurements showed that two composites consisted of a normal -tricalcium phosphate structure and bone-like apatite formation on their surface. When cells were cultured on two scaffolds, no proliferation inhibition was found. Osteoblasts attachment of sample 2 was significantly better than that of sample 1 after 1 and 3 days of culture. These results suggest that two composites are promising materials for bone repairing and provide further basis for the use of -tricalcium phosphate/ apatite composite as the scaffold in bone tissue engineering.
%K &beta
%K -tricalcium phosphate
%K Bone-like apatite
%K Characterization
%K Biocompatibility<br>β-磷酸三钙
%K 类骨磷灰石
%K 结构特征
%K 生物相容性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=581A0A94E0F45BAC&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=38B194292C032A66&sid=EF27C460877D3C9F&eid=D2742EEE6F4DF8FE&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=17