%0 Journal Article
%T USE OF Tb~(3+) AS A LUMINESCENT PROBE TO STUDY THE INTERACTION OF CALMODULIN WITH ITS ANTAGONISTS<br>Tb~(3+)作为荧光探针研究钙调蛋白与拮抗药物的相互作用
%A 区耀华
%A 王志刚
%A 周昕
%J 生物物理学报
%D 1990
%I 
%X A simple, rapid and sensitive method determining the binding sites of antagonists on calmodulin was introduced.Studies on the intrinsic fluorescence of calmodulin have shown that Tb3+ can efficiently bind to the Ca2+ -binding sites on calmodulin and then induce conformational changes similar to those of Ca2+. Because each of domains III and IV on calmodulin has one tyrosine, the binding of Tb3+ to calmodulin was followed by obvious increase of Tb3+ fluo-rescence at 490 nm and 545nm, which was caused by exciting the calmodulin tyrosine at 280 nm with energy transfer from tyrosine to Tb3+ . By studing the effect of calmodulin antagonists on this fluorescence, it was found that the binding sites of TEP were located on the C-terminal part of calmodulin which contains the domains III and IV; whereas the binding sites of jujuboside A were located on the N-terminal part which contains the domains I and II.
%K 钙调蛋白
%K 拮抗药
%K Tb^3＋
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=33065D87B9A63FA37BB93FDA06244A32&yid=8D39DA2CB9F38FD0&vid=B31275AF3241DB2D&iid=38B194292C032A66&sid=B1E36BF7B9783A85&eid=407C905D8F0449C4&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=3&reference_num=1