%0 Journal Article
%T Expression of Goat Follicle-stimulating Hormone Analogous Gene in Pichia pastoris<br>山羊重组促卵泡素长效类似物基因在毕赤酵母中表达
%A Huanli Yang
%A Xibiao Zhao
%A Xiaojuan Song
%A Jiatong Ding
%A <br>杨欢利
%A 赵西彪
%A 宋晓娟
%A 丁家桐
%J 生物工程学报
%D 2008
%I 
%X In order to obtain the long-acting FSH preparation, the single strand long-acting analogous gene FSHbeta-CTP-alpha was successfully constructed by the C-terminal peptide(CTP) of carboxyl-terminal region of human chorionic gonadotropin with the goat FSHalpha-subunit and beta-subunit genes, then it was inserted into pPIC9K vector. The recombinant plasmid pPIC9K FSHbeta-CTP-alpha was transformed into Pichia pastoris GS115 by electroporation. The multi-copy inserts His+Mut+ were gained by the screening of phenotype and hyper-resistance to G418. After methanol induction, the supernatant was analysised by SDS-Polyacrylamide Gen Electrophoresis and Western blot. The results show that the transformants of FSHbeta-CTP-alpha could express the objective protein successfully and the molecular weight is about 29 kD. The concentration of supernatant was detected by Radio-immunoassay and the average expression of multi-inserts is 91.849 mIU/mL and the low-inserts is 37.419 mIU/mL. The expression of multi-inserts is higher than the low-inserts significantly. This research lay the foundation for studying the structure of FSH and the production of long-acting FSH preparation.
%K follicular-stimulating hormone
%K long-acting analogous gene
%K Pichia pastoris<br>山羊促卵泡素
%K 长效类似物基因
%K 毕赤酵母
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6D0A3A4146D6CFECA221EB0775A26A1D&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=0493D643315CD829&eid=3081401A9FAB9CE2&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=25