%0 Journal Article
%T Agrobacterium rhizogenes-mediated Transformation and Regeneration of the Apocynum venetum<br>纤维植物罗布麻发根的诱导及植株再生
%A Haiyan Ji
%A Bing Zhao
%A Xiaodong Wang
%A Yuchun Wang
%A <br>贾海燕
%A 赵兵
%A 王晓东
%A 王玉春
%J 生物工程学报
%D 2008
%I 
%X A system for the Agrobacterium rhizogenes-mediated transformation and plant regeneration of A. venetum has been developed. The highest transformation frequency was 100%, achieved by using strain LBA9402 with root explants. The highest density of hairy roots reached 22 when root explants transformed by R1000 cultured in the dark. Adventitious shoots were obtained from profusely branched, fast-growing (type PBF) hairy roots, and the adventitious shoot induction frequency was 20%. Regenerated shoots rooted easily on hormone-free 1/2 MS solid medium in 2 weeks. Approximately 1/3 regenerated plants derived from hairy roots exhibited prolific roots with shortened internodes. Whereas other regenerated plants showed another phenotype: long internodes, strong stems, and fleshy blades. However, all regenerated plants displayed a relatively fast development procedure and stronger than the aseptic seedlings. Polymerase chain reaction (PCR) analyses confirmed the hairy root lines and regenerated plants were induced by A. rhizogenes.
%K Apocynum venetum
%K Agrobacterium rhizogenes
%K hairy roots
%K plant regeneration
%K transformation<br>罗布麻
%K 发根农杆菌
%K 发根
%K 植株再生
%K 转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2C93B71714A71343AC086269CC9A74B2&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=F3090AE9B60B7ED1&sid=31F5E7613DCE2981&eid=D2FD077E5EE423CA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15