%0 Journal Article
%T Construction of Aspergillus niger lipase mutants with oil-water interface independence<br>不依赖油水界面激活的黑曲霉脂肪酶突变体的构建
%A De Chen
%A Zhengyu Shu
%A Longyin Xue
%A Ruifeng Lin
%A Jiguang Wu
%A Yongmei Jiang
%A Xin Li
%A Yuexin Lin
%A Jianzhong Huang
%A <br>陈的
%A 舒正玉
%A 薛龙吟
%A 林瑞凤
%A 吴继光
%A 蒋咏梅
%A 李欣
%A 林跃鑫
%A 黄建忠
%J 生物工程学报
%D 2011
%I 
%X Based on previous bioinformational analysis results, two Aspergillus niger lipase (ANL) mutants, ANL-Ser84Gly and ANL-Asp99Pro were constructed to screen ANL mutants with oil-water interface independence. ANL-Ser84Gly still displayed a pronounced interfacial activation, while ANL-Asp99Pro displayed no interfacial activation. The specific activity of ANL-Ser84Gly towards p-nitrophenyl palmitate (-myristate, -laurate and -decanoate) decreased by 29.8% (53.1, 60.1 and 77.1, respectively) than that of ANL, while the specific activity of ANL-Asp99Pro towards p-nitrophenyl palmitate increased by 2.2-fold. The mutation in the hinge region at both sides of the lid domain also destabilized various secondary structure factors of ANL-S84G and ANL-D99P, which resulted in a substantial decrease in thermostability. The achievement to construct oil-water interface-independent ANL mutants would help to further understand lipase interfacial activation mechanism.
%K Aspergillus niger
%K lipase
%K lid domain
%K interfacial activation
%K overlap extension polymerase chain reaction<br>黑曲霉，脂肪酶，盖子结构域，界面活性，重叠延伸聚合酶链式反应
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9880CD65C317F63DEF178B6E0693A8E9&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=E9F71A2A3584AD5D&eid=65A51D0EBEB846F5&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0