%0 Journal Article
%T Transfecting Keratinocyte and Enhancing Cell Proliferation by Integrated HPV11 DNA<br>乳头瘤病毒11型完整基因组成功转染角质形成细胞并促其增殖
%A Yi Zhang
%A Fenglin Lü
%A Caiyu Chen
%A <br>张一
%A 吕凤林
%A 陈彩宇
%J 生物工程学报
%D 2008
%I 
%X We constructed the plasmid containing the integrated open reading frame (ORF) HPV11 genome, and identified its function, to lay foundation for production of transgenic animal models of HPV11. Recombinant plasmid pQE-Trisystem- EGFP/HPV11 (pE/H) and pQE-Trisystem-EGFP/1.1 copy HPV11 (pE/1.1H) were constructed. Then, the human keratinocyte (KC) was transfected by pE/1.1H, pE/H and closed circular HPV11 and detected. The recombinant plasmid was successfully constructed. The expression of HPV11E6 gene was detected in the experimental group. Fluorescence was observed in the pE/1.1H and pE/H group. The HPV11, pE/H, and pE/1.1 enhanced the KC proliferation, with remarkable differences (P<0.01) from the control group. Amongst the three experimental groups, pE/1.1H was found to be the weakest. The plasmid containing the integrated ORF of HPV11 (1.1 copy HPV11) genome was successfully constructed. The pE/1.1H had the same phenotype of wild-type HPV11 genome. It provided experimental material and methodological guidance for studying the low-risk HPV, as well as for the production of HPV11 transgenic mice.
%K HPV11
%K keratinocyte
%K cell proliferation<br>人乳头瘤病毒11型
%K 角质形成细胞
%K 细胞增殖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F92C462D20412687BB04F63116AFBAF6&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=5D311CA918CA9A03&sid=7264799D7F648D02&eid=40295B54D40AE4B3&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11