%0 Journal Article
%T Pre-L-methionine Feeding Strategy for S-adenosyl- L-methionine Fermentative Production<br>发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略
%A Jiepeng Wang
%A Tianwei Tan
%A <br>王杰鹏
%A 谭天伟
%J 生物工程学报
%D 2008
%I 
%X The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively.
%K S-adenosyl-L-methionine
%K saccharomyces cerevisiae
%K high-cell-density fermentation
%K L-methionine<br>S-腺苷蛋氨酸
%K 酿酒酵母
%K 高密度发酵
%K L-蛋氨酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3DC3F6CBE9F8CEA2987EA3CB173D354E&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=F3090AE9B60B7ED1&sid=4832190D43CC78F4&eid=06AAA405D95B59CF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=9