%0 Journal Article
%T Screening of T7 Phage-display cDNA Library from Liver of Microtus fortis with Extracts of Schistosomulum and Characterization of the Positive Clones<br>日本血吸虫童虫裂解物对东方田鼠肝脏T7噬菌体展示cDNA文库的筛选及阳性克隆分析
%A Renchu Ji
%A Yi Sun
%A Jinming Liu
%A Zhiqiang Fu
%A Chunxiu Yuan
%A Yaojun Shi
%A Ke Lu
%A Huan Sun
%A Hao Li
%A Yamei Jin
%A Jiaojiao Lin
%A <br>贾人初
%A 孙毅
%A 刘金明
%A 傅志强
%A 苑纯秀
%A 石耀军
%A 陆珂
%A 孙焕
%A 李浩
%A 金亚美
%A 林矫矫
%J 生物工程学报
%D 2008
%I 
%X Microtus fortis is naturally resisitent to Schistosoma japonicum. In order to find schistosome-resistence-related genes of Microtus fortis, a T7 phage-display cDNA library from liver of Microtus fortis was screened with the soluble lysate of schistosomula. The specific phages were enriched 375-fold after 3 rounds of biopanning. Ninety-two positive clones picked at random were sequenced and 19 ESTs including 6 unreported genes were obtained. Compared with the negative phage clone control, five positive clones, No.4 (GenBank Accession No.: EW968294), No.13 (GenBank Accession No.: EW968303), No.14 (GenBank Accession No.: EW968304), No.15 (GenBank Accession No.: EW968305) and No.18 (GenBank Accession No.: EW968308) could induce significantly higher schistosomula mortality rate when co-cultivated with schistosomula. According to the function analysis and the shistosomula-killing effect in vitro, the genes encoding CASP8 and FADD-like apoptosis regulator isoform protein, alpha-2-HS-glycoprotein, M4 protein, R3H domain (binds single-stranded nucleic acids) isoform 2 and 3 previously unreported proteins (No.14, No.15 and No.18) obtained here, were schistosomiasis-resistence-related genes of Microtus fortis.
%K Schistosoma japonicum
%K Microtus fortis
%K liver
%K T7 phage-display cDNA library
%K schistosomia-resistence-related gene<br>日本血吸虫
%K 东方田鼠
%K 肝脏
%K 噬菌体展示cDNA文库
%K 抗性基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7E144631A56CF49002816DFDEEE5A0E5&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=EED44B83FAB71309&eid=547991B346A6585E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12