%0 Journal Article
%T Construction and expression of disulphide stabilized anti-CD3/anti-Pgp diabody<br>二硫键稳定的抗CD3/抗Pgp微型双功能抗体的构建、表达及活性测定
%A Ye Su
%A Juanni Liu
%A Yingdai Gao
%A Li Qin
%A Ming Yang
%A Jinhong Wang
%A Yuanfu Xu
%A Xiaofeng Shao
%A Qing Ji
%A Dongsheng Xiong
%A Chunzheng Yang
%A <br>苏晔
%A 刘娟妮
%A 高瀛岱
%A 秦立
%A 杨铭
%A 王金宏
%A 许元富
%A 邵晓枫
%A 纪庆
%A 熊冬生
%A 杨纯正
%J 生物工程学报
%D 2009
%I 
%X We constructed and expressed an anti-CD3/anti-Pgp (P-glycoprotein) diabody previously. However, the two chains of diabody are associated non-covalently, resulting in being capable of dissociating. The aim of this study is to enhance the stability of the diabody. We introduced cysteine residues into the CD3 or Pgp V-domain to covalently lock the two chains together. The disulphide crosslinked diabody were expressed by Escherichia coli (E. coli) 16C9 and purified by a cation exchange column and an anti-Etag a...
%K diabody
%K disulphide bond
%K drug stability
%K engineering antibody<br>基因工程抗体
%K diabody
%K 二硫键
%K 药物稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=26BDE5DC6766FF70C6642663D362FB30&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=DF92D298D3FF1E6E&sid=9E5DBB98644FDC52&eid=94D812A784CFA7CC&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17