%0 Journal Article
%T Gene expression profiles of growth and reproduction related genes during the early development of common carp (Cyprinus carpio L.)<br>鲤鱼发育早期HPG轴和GH/IGF轴相关因子的转录起始分析
%A LU Yue-Jiao
%A HU Wei
%A ZHU Zuo-Yan
%A <br>卢月娇
%A 胡炜
%A 朱作言
%J 水生生物学报
%D 2009
%I 
%X As many researches revealed some relations between HPG and GH/IGF axis in teleost, we detected gene expression profiles of growth- and reproduction-related genes from HPG or GH/IGF axis in common carp ( Cyprinus carpio) embryos and early larval stages, in order to find some regulatory relations between HPG and GH/IGF axis in the early development stages of common carp. The genes under detection were sGnRH, cGnRH, GtH α subunit and β subunit from HPG axis and GH, IGF, growth hormone receptor (GHR) from GH/IGF axis, with β-actin gene as the internal control. Samples were collected from embryos and larvae of unfertilized eggs, 64-cell stage, 32-cell stage, 256-cell stage, 50%-epiboly, 20-, 23-, 26-, 32-, 40-, 46- and 58- hours post fertilization ( hpf) , and 1-, 2-, 3-, 5-, 7- and 9- days post hatching (dph) reared at 23℃, and then RNA extracted from the samples was used in RT-PCR. sGnRH, cGnRH, GtH α subunit, GtH-I β subunit, GtH-II β subunit, GH, GHR and IGF-1 showed different expression patterns in carp embryos and larvae. Development around 20h post fertilization ( hpf) and 23hpf was critical for carp embryos, as sGnRH, cGnRH, GtH-1β subunit and GHR were first detectable at 20hpf, IGF-1 at 23hpf and GtH-II β at 26hpf. Meanwhile, embryos in 20hpf and 23hpf stages showed apparent development in brain region, indicating a regulatory function of GnRH and GtH in the development of brain. GtH α subunit was first detectable at 46hpf while GH was first detectable at 1 d post hatching (dph). The difference in the timing of the expression of GH and IGF mRNA may suggest that IGF gene expression is not GH-dependent. Additionally, the timing of the expression of GtH α subunit, GtH-I β subunit, GtH-II β subunit were unsyn-chronized in embryos, suggesting a regulatory function of GtH α subunit in GtH expression.
%K GnRH/GtH
%K GH/IGF/GHR<br>鲤鱼胚胎
%K 转录起始
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=72FDB2EDF2DC2608731445ED2F347975&yid=DE12191FBD62783C&vid=27746BCEEE58E9DC&iid=B31275AF3241DB2D&sid=2B25C5E62F83A049&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=2&reference_num=38