%0 Journal Article
%T Cloning of human lysosomal acid b-glucosidase gene and its expression in COS7 cells<br>人溶酶体酸性b-葡萄糖脑苷脂酶基因的克隆及其在COS7细胞中的表达
%A Yanli Zhang
%A Dan Xu
%A Ziyu Wang
%A Li Meng
%A Feng Wang
%A <br>张艳丽
%A 许丹
%A 王子玉
%A 孟立
%A 王锋
%J 生物工程学报
%D 2009
%I 
%X In this study, we amplified human lysosomal acid b-glucosidase (GlcCerase) gene by RT-PCR from human placenta, and analyzed the sequence of the PCR product cloned in pMD-19T. The gene identity was 99% comparable to that of the reported human GlcCerase cDNA sequence in GenBank. The GlcCerase gene digested with Xho I was subcloned into eukaryotic express vector pEGFP-C1 to generate recombinant expression vector pEGFP-GlcCerase. After identified the recombinant plasmid by restriction enzyme digestion, we transfected pEGFP-GlcCerase into COS7 cells by liposome. GlcCerase mRNA was expressed and the activity of GlcCerase was also detected in COS7 cells. This study would lay a foundation for the function of GlcCerase and its production by transgenic bioreactor.
%K GlcCerase gene
%K cloning
%K eukaryotic expression vector
%K COS7 cells
%K transfection<br>人溶酶体酸性b-葡萄糖脑苷脂酶基因
%K 克隆
%K 真核表达载体
%K COS7细胞
%K 转染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=665339FC7763160385D77C0DAED3A5AF&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=96A53C367B5173D7&eid=866F8A6B640835A7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0