%0 Journal Article
%T Development in ligase-mediated techniques for bio-molecular analysis<br>连接酶介导的生物分子检测技术
%A 许超
%A 周亚凤
%A 张先恩
%J 生物工程学报
%D 2009
%I 
%X Two oligonucleotide probes are permitted to anneal to the nucleic acid target of interest so that the ends of two probes immediately become adjacent to each other. The ligase can then efficiently join the two juxtaposed oligonucleotide probes by the formation of a phosphodiester bond if and only if perfectly matched base-pairs at the nick are present. During past 20 years, many ligase-mediated techniques have been developed for analyzing various bio-molecules, such as known/unknown point mutations, small-sc...
%K ligase
%K nucleic acid polymorphic variation
%K bio-molecular interaction
%K detection<br>连接酶
%K 核酸多态性
%K 生物分子相互作用
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=AD0C878F62589CEFFF53C11569A828D1&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=38B194292C032A66&sid=A5B34D9E8FDA439A&eid=B66C5792F4740920&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=34