%0 Journal Article
%T Cholesterol depletion up-regulates voltage-dependent K+ current in rat hippocampal neurons<br>海马神经细胞胆固醇含量降低对其电压依赖钾电流的上调作用
%A GUO Jian-li
%A YANG Zeng-qi
%A QI Zhi
%A <br>郭建立
%A 戚智
%A 杨增岐
%J 生物物理学报
%D 2006
%I 
%X Cholesterol is ubiquitous in cell membranes and its content changes during development, aging or in various diseases, this means that it plays an important role in modulating cell functions. This study investigated whether changes in cholesterol content could modulate activity of the voltage-dependent K+ (Kv) current in rat hippocampal neurons by using whole cell patch clamp technique. Acutely depletion of membrane cholesterol by exposing the cells to methyl-beta-cyclodextrin (MβCD), a cholesterol-sequestering molecule, resulted in an enhancement of IK (delayed rectifier potassium current) and delaying the inactivation of IA (transient outward potassium current). Furthermore, cholesterol depletion almost had no effect on IK and IA current after treatment with TEA and 4-AP on the cells, respectively, indicating that the effect of membrane cholesterol on up-regulation of the Kv current was due to the activation of both IK and IA currents. In view of important roles of IK and IA currents in the neuronal functions, these results suggest that cholesterol may play a regulatory role in the neuronal excitability.
%K Cholesterol
%K Hippocampal neuron
%K Methyl-&beta
%K -cyclodextrin
%K Voltage-dependent potassium channel<br>胆固醇
%K 海马神经细胞
%K β-甲基环化糊精
%K 电压依赖钾通道
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=30CFBC936DB1B053&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=BA79719BCA7341D5&eid=31611641D4BB139F&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=45