%0 Journal Article
%T Purification of recombinant lysostaphin by monoclonal antibody affinity chromatography<br>单克隆抗体亲和层析法纯化重组溶葡萄球菌酶
%A Jinjiang Huang
%A Hongyu Wu
%A Jien Zhang
%A Qingshan Huang
%A <br>黄晋江
%A 吴宏宇
%A 张继恩
%A 黄青山
%J 生物工程学报
%D 2009
%I 
%X Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant. This study established monoclonal antibody affinity chromatography to obtain lysostaphin of high purity for drug-use standard. The purified Lysostaphin was of > 95% purity and its recovery rate more than 90%. Moreover, the affinity column kept its efficiency of purification invariable after more than 30 times repeat. Also, the dye release assay validated that the purified lysostaphin had significant bactericidal activity. This method was simple and of high efficacy for the lysostaphin purification and showed its potency in commercial production.
%K lysostaphin
%K monoclonal antibody
%K affinity chromatography<br>溶葡萄球菌酶
%K 单克隆抗体
%K 亲和层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7A169462F25BB12BBF7D70247283EA91&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=CCFEF18AD66F9995&eid=7ACC6238C6DFEBD4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=16