%0 Journal Article
%T Effect of mufti-gene knockout of L-tryptophan transport system on L-tryptophan production in Escherichia coli<br>大肠杆菌色氨酸转运系统多基因敲除对色氨酸生产的影响
%A Zhijun Zhao
%A Sheng Chen
%A Dan Wu
%A Jing Wu
%A Jian Chen
%A <br>赵志军
%A 陈晟
%A 吴丹
%A 吴敬
%A 陈坚
%J 生物工程学报
%D 2011
%I 
%X In Escherichia coli, uptake of L-ryptophan is done by three distinct permeases, encoded by mtr, tnaB, and aroP. Based on the mtr single-gene knockout, we constructed the mtr.tnaB and mtr.aroP double-gene knockout mutants and the mtr.tnaB.aroP triple-gene knockout mutant. The fermentation results showed that the mtr.tnaB and mtr.aroP knockout mutants produced 1.38 g/L and 1.27 g/L L-ryptophan, respectively, which was 17% and 9% higher than that of the mtr knockout mutant. However, the mtr.tnaB.aroP knockout mutant was significantly affected on cell growth and only produced 0.63 g/L L-ryptophan. During the fed-batch fermentation in a 3-L fermentor, the mtr.tnaB knockout mutant produced 12.2 g/L L-ryptophan, which was 27% higher than that of the mtr knockout mutant. This study demonstrates the effect of multi-gene knockouts of L-ryptophan transport system of Escherichia coli on the biosynthesis of L-ryptophan.
%K transport system
%K knockout
%K L-tryptophan
%K Escherichia coli<br>转运系统
%K 基因敲除
%K L-色氨酸
%K 大肠杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=042A5D990BB41D61FAD134C4BAEA1221&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=59906B3B2830C2C5&sid=0B2B64BA93B3A889&eid=314F4A89DDA6AB41&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=19