%0 Journal Article
%T Increasing activity of Rhizopus chinensis (CCTCC M201021) lipase by directed evolution-error prone PCR<br>定向进化-易错PCR方法提高华根霉Rhizopus chinensis CCTCC M201021脂肪酶的活力
%A Rui Wang
%A Xiaowei Yu
%A Chong Sha
%A Yan Xu
%A <br>王睿
%A 喻晓蔚
%A 沙冲
%A 徐岩
%J 生物工程学报
%D 2009
%I 
%X Directed evolution strategy (error-prone PCR) was conducted to improve the activity of lipase from Rhizopus chinensis CCTCC M201021.Through two rounds of ep-PCR and pNPP top agar screening,two optimum mutant strains 1-11 and 2-28 were obtained with 2 and 4 fold of enzyme activity higher than that of parent strain,respectively.DNA sequencing of mutant lipase 2-28 revealed four amino acid substitutions:A129S,K161R,A230T,K322R.According to the simulated protein structure of Rhizopus chinensis lipase,A129S,K161...
%K directed evolution
%K error-prone PCR
%K Rhizopus chinensis lipase
%K lipase activity<br>定向进化
%K 易错PCR
%K 华根霉脂肪酶
%K 脂肪酶活力
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DF3D14D9FECBB13484F91DE6F1F4232A&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=59906B3B2830C2C5&sid=22AA0D3D54DBF917&eid=5349C9BD3C454FC9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=12