%0 Journal Article
%T Screening and characterization of lipase from a metagenome library of dairy rumen microflora<br>奶牛瘤胃微生物元基因组文库中脂肪酶的筛选与酶学性质
%A Shengguo Zhao
%A Jiaqi Wang
%A Kailang Liu
%A Yaxin Zhu
%A Dengpan Bu
%A Dan Li
%A Ping Yu
%A <br>赵圣国
%A 王加启
%A 刘开朗
%A 朱雅新
%A 卜登攀
%A 李旦
%A 于萍
%J 生物工程学报
%D 2009
%I 
%X Using lipase segregation agar containing trioleoylglycerol,we obtained 18 lipase positive clones by screening from a metagenome library of dairy rumen microflora containing 15 360 clones.The average insert size of lipase positive clones was about 60 kb.Lipase enzyme activity analysis by p-NPP method indicated that Lipase6,Lipase7 and Lipase8 had higher lipolytic activities to substrates of p-nitrophenyl palmitate(C16),p-nitrophenyl alaurate(C12) and p-nitrophenyl palmitate(C16) respectively.The optimum pH o...
%K rumen bacterium
%K metagenome library
%K lipase
%K substrate
%K optimum pH
%K thermo stability<br>瘤胃微生物
%K 元基因组文库
%K 脂肪酶
%K 底物特异性
%K 最适pH
%K 热稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=940059F99750DC0EC65202DF72CB6C7B&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=F26986CDF689DBC4&eid=3893EBCAC6700388&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=18