%0 Journal Article
%T Cloning, Expression and Antiviral Activity of Arctic Fox (Alopex lagopus) Interferon-g Gene<br>北极狐g-干扰素cDNA的克隆、表达及活性测定
%A Hailing Zhang
%A Xiuli Chai
%A Guoliang Luo
%A Fengxue Wang
%A Li Yi
%A Xiqun Shao
%A Xijun Yan
%A <br>张海玲
%A 柴秀丽
%A 罗国良
%A 王凤雪
%A 易立
%A 邵西群
%A 闫喜军
%J 生物工程学报
%D 2008
%I 
%X In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-g), We have isolated the cDNA encoding arctic fox(Alopex lagopus)VuIFN- g. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.4% for nucleotide identity with silver fox and canine, respectively, and 100% for amino acid identity. Expression of recombinant mature arctic fox interferon gamma (mVuIFN-g) in bacterial system was confirmed by SDS-PAGE and Western blotting analysis. Recombinant VuIFN-g showed higher antiviral activity against vesicular stomatitis virus in cultured Vero and MDCK by inhibiting virus induced cytopathic effect, In view of the immunomodulatory and antiviral activities of VuIFN-g, it may provide a basis for further research on antiviral therapy of recombinant VuIFN-g in economic animal practice.
%K Arctic fox
%K interferon gamma
%K antiviral activity<br>北极狐
%K 干扰素-g
%K 抗病毒活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E1BDF3916EAB95A07DFBC911757F5D92&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=9CF7A0430CBB2DFD&sid=D2F1C862439505C0&eid=2AAEC1E50A125188&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0