%0 Journal Article
%T High-yielding cordycepin in Cordyceps militaris modified by low-energy ion beam<br>低能离子束修饰蛹虫草菌株高产虫草素
%A Wen Li
%A Shiguang Zhao
%A Hongwei Chen
%A Hang Yuan
%A Tao Wang
%A Xian Huang
%A <br>李文
%A 赵世光
%A 陈宏伟
%A 袁航
%A 王陶
%A 黄献
%J 生物工程学报
%D 2009
%I 
%X Cordycepin has the effect of anti-tumor, immunomodulation, anti-inflammation and so on. In order to make use of Cordyceps militaris better, we implanted different doses of low-energy ion beam into C. militaris, chose best cordycepin extracting technology , and determined cordycepin content in strains before and after ion beam implantation by UV spectrophotometry. Results showed that the best dose of low-energy ion beam was 2.60×1015 ions/cm2, the best conditions of microwave assisted ultrasonic extraction technology were as follows: 70% ethanol as the solvent, microwave power as 200 W, extraction time as 110 s, Material - liquid ratio as 1: 240. We chose 15 strains with high-yielding cordycepin, of which cordycepin content was up to (11.924±0.063)?mg/g, which was a nearly 30% increase compared with the original strain.
%K Cordyceps militaris
%K cordycepin
%K low-energy ion beam
%K modification<br>蛹虫草
%K 虫草素
%K 低能离子束
%K 修饰
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8EC8ECD1E35C5CB88F4B5797434069BF&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=708DD6B15D2464E8&sid=7DFC61BD88FB3757&eid=BF631CA8030AF011&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=21