%0 Journal Article
%T Screening, Identifying of Cellulose-Decomposing Strain L-06 and Its Enzyme-Producing Conditions<br>纤维素降解菌L-06的筛选、鉴定及其产酶条件的分析
%A Yuntao Liu
%A Shuxia Xuan
%A Chuannan Long
%A Minnan Long
%A Zhong Hu
%A <br>刘韫滔
%A 禤淑霞
%A 龙传南
%A 龙敏南
%A 胡忠
%J 生物工程学报
%D 2008
%I 
%X Cellulases are relatively costly enzymes that are sold in large volumes for use in different industrial applications, and a significant reduction in cost will be important for their commercial use in biorefineries. The production of cellulase is a major factor in the hydrolysis of cellulosic materials. Hence it is essential to make the process economically viable. A strain (L-06) with high cellulase activity was screened from rice straw compost and classified as Penicillium decumbens by the analysis of its morphology and 18S rRNA gene sequences. Different conditions of liquid fermentation medium including nitrogen source, carbon source, surfactant, temperature, initial pH, inoculation quantity for the production of cellulase had been studied. The maximal b-1, 4-glucosidase(BGL) activity was 1662 u/mL which is 1.49 times of the previous and the maximal exo- b-1, 4-glucanases(CBH) activity was 2770 u/mL which is 1.36 times of the previous, cultured in the optimal condition for three days. And the maximal endo-b-1, 4-glucanases (EG) activity was 18064 u/mL which is 1.87 times of the previous and the maximal filter paper enzyme(FPase) activity was 4035 u/mL which is 1.47 times of the previous , cultured in the optimal condition for four days. In the optimization experiments, the EG and CBH in the culture condition (pH10) maintained 70% and 43% activity. In the culture condition(50oC) EG and CBH maintained 59% and 68% activity, which showed heat and alkali resistant characteristics.
%K cellulase
%K Penicillium decumbens
%K cultural condition optimization<br>纤维素酶
%K 斜卧青霉
%K 培养条件优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4B15167F747652919B604EFD56956A58&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=1AA557EFF1C6B447&eid=76C32027E03E49D7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=1