%0 Journal Article
%T Expression, purification and crystallization of human DOC-1R<br>人口腔癌症缺失相关蛋白（DOC-1R）的表达、纯化和晶体生长
%A WANG jie
%A CHENG Zhong-jun
%A GONG Wei-min
%A <br>王杰
%A 程中军
%A 龚为民
%J 生物物理学报
%D 2006
%I 
%X DOC-1(Deleted in Oral Cancer-1)is a growth suppressor gene identified first in oral cancer.DOC-1R(DOC-1 related)gene is another tumor suppressor gene which was cloned in 1999.Recent studies suggested that DOC-1 and DOC-1R share similarity in both of their sequences and functions.However,there is no homology structure found in PDB.Human doc-1r gene was cloned into expression vector pET-22b( )and over-expressed in E.coli strain BL21(DE3).Human DOC-1R protein was purified in a two-step procedure of Ni-NTA affinity followed by Superdex 75 chromatography.Over 98% of purified DOC-1R proteins was finally got for structural and functional research.Mass Spectrum identified that the exact molecular weight of DOC-1R is 14091.23 Da;Dynamic light scattering assay showed that its homogenesis was over 99.0%.The crystals of DOC-1R were obtained by using hanging-drop vapor-diffusion method.
%K DOC-1R
%K Expression
%K Purification
%K Mass spectrum
%K Dynamic light scattering
%K Crystallization<br>口腔癌症缺失
%K DOC-1R
%K 表达
%K 纯化
%K 质谱
%K 动态光散射
%K 晶体生长
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=94A32D8DE9F4D4A6&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=38B194292C032A66&sid=D2742EEE6F4DF8FE&eid=CEC789B3C68C3BB3&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=9