%0 Journal Article
%T Isolation and identification of a methanogen from the high temperature oil reservoir water<br>高温油藏采出液中嗜热产甲烷古菌的分离鉴定
%A Haichang Liu
%A Guihong Lan
%A Quanquan Liu
%A Wenjing Zhang
%A Yi Cao
%A Yu Deng
%A Hui Zhang
%A <br>刘海昌
%A 兰贵红
%A 刘全全
%A 张文静
%A 曹毅
%A 邓宇
%A 张辉
%J 生物工程学报
%D 2010
%I 
%X To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60°C, pH 7.0?7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364).
%K microorganisms in petroleum reservoir
%K thermautotrophic methanogen
%K phylogenetic analysis<br>油藏微生物，热自养产甲烷杆菌，系统发育分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E686B397B13A2F557D4527C0B2011721&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=DF92D298D3FF1E6E&sid=9F481C73BF82C48F&eid=4D5C5090B0F83CF9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0