%0 Journal Article
%T In vitro transgenic expression efficacy of a helper-dependent adenoviral vector encoding enhanced green fluorescent protein<br>辅助病毒依赖型腺病毒载体转基因的体外表达效率
%A Xianxian Zheng
%A Jinsheng He
%A Yuanhui Fu
%A Shaohua Xu
%A Can Xie
%A Changxin Shi
%A Mei Zhang
%A Xiaobo Wang
%A Tao Hong
%A <br>郑娴娴
%A 何金生
%A 付远辉
%A 徐少华
%A 谢灿
%A 石长信
%A 张梅
%A 王小波
%A 洪涛
%J 生物工程学报
%D 2010
%I 
%X To investigate the transgenic expressing efficacy of helper-dependent adenoviral vector (HDAd) in vitro, we constructed a HDAd encoding enhanced green fluorescent protein (EGFP), denominated as HDAd/EGFP, performed large scale preparation and purification, and then identified the purified HDAd/EGFP under fluorescent microscope and electron microscope. After the concentration of HDAd/EGFP was determined by spectrophotometer, the transgenic expression efficiency of HDAd/EGFP was compared with first generation adenoviral vector encoding EGFP (FGAd/EGFP) in vitro. Therefore, we infected A549 cells with 2 000 virus particles (vp) per cell by HDAd/EGFP and FGAd/EGFP respectively and analyzed EGFP expressing level by flow cytometry. Consequently, the fluorescent expression rate and fluorescent intensity of EGFP were higher in infected A549 cells by HDAd/EGFP than by FGAd/EGFP. HDAd, capable of expressing transgene instantly and efficiently in vitro, is a potential vaccine vector.
%K helper-dependent adenoviral vector (HDAd)
%K first generation adenoviral vector (FGAd)
%K enhanced green fluorescent protein (EGFP)
%K transgenic expression<br>辅助病毒依赖型腺病毒载体，第一代腺病毒载体，增强型绿色荧光蛋白，转基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=103107162C278511662C4D1FFD4C8947&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=B3BA5701971F4D8F&eid=CA10C709B736BBEA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0