%0 Journal Article
%T AN INVESTIGATION OF CONFIGURATION OF ERYTHROCYTE MEMBRANE PROTEINS WITH MALEIMIDE SPIN LABELS DURING BLOOD PRESERVATION<br>用马来酰亚胺自旋标记研究库存血红细胞膜蛋白质构象
%A 吴泽志
%A 陈槐卿
%A 薛振南
%A 石红联
%A 赵保路
%A 忻文娟
%J 生物物理学报
%D 1992
%I 
%X Two maleimide spin labels, malcimide I and maleimide V, were used to investigate the dynamic changes of the conf ipura t ion and sulfhydryl binding sites of erythrocyte membranc protein- within a 35-day period of ACD-B blood preserved. The results showed: during the preservation period, the S/W ratio ealculated from ESR spectra of maleimide I-labelled erythrocytes decreased rapidly to a lower level; the rotational eorrtlation time (t,.) inferred from ESR spectra of maleimide V-lahelled erythrocytes decreased rapidly in the first week but increased slowly to the initial level in the later stage. The decrease of S /W ratio demonstrated configuration changes of erythrocyte membrane proteins. The changes of rotational correlation time might reflect that the micro-viscosity in the superficial aqueous portion around the membrane proteins,perhaps including the adjacent superficial lipid environment ,undcrwent a bipha-sic change, i.e, decreased rapidly then increased slowly during preservation.
%K blood preservation
%K crythrocyle membrane
%K maleimide spin labels<br>血液保存
%K 红细胞膜
%K 蛋白
%K 构象
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=6F4B2AF07746A9C451F2BAAAA46CA76C&yid=F53A2717BDB04D52&vid=5D311CA918CA9A03&iid=E158A972A605785F&sid=507521DBC725630F&eid=7F9B7E84827A650F&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=1