%0 Journal Article
%T Construction of Dunaliella salina heterotrophic expression vectors and identification of heterotrophically transformed algal strains<br>杜氏盐藻异养表达载体的构建及异养转化株的鉴定
%A 陈涛
%A 刘红涛
%A 吕鹏举
%A 薛乐勋
%J 生物工程学报
%D 2009
%I 
%X We constructed inducible and constitutive heterotrophic expression vectors of Dunaliella salina (D. salina) and identified heterotrophic transformants. A gene encoding a glucose transporter (Glut1) was cloned from human placenta tissues by RT-PCR and sequenced. Inducible heterotrophic expression vector pMDDGN-Bar of D. salina, which included a duplicated carbonic anhydrase (DCA) promotor and a Bar selectable marker that could drive expression of the Glut1 gene in D. salina, was constructed by molecular biol...
%K Dunaliella salina
%K expression vector
%K heterotrophy
%K glucose transporter1(Glut1)<br>杜氏盐藻
%K 表达载体
%K 异养
%K 葡萄糖转运基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E4EC18A5F1974031CA97ABE4D40866C1&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=38B194292C032A66&sid=5A735990D5DE8BF4&eid=4F0B2F798E08B761&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=7